Production and Characterization of an Alkaline Lipase from Thermophilic Anoxybacillus sp. HBB16
نویسندگان
چکیده
منابع مشابه
Production, purification and characterization of thermophilic lipase from Bacillus sp. THL027.
A low-cost medium, MGRS, has been developed for growth and lipase production from Bacillus THL027 at 65 degrees C and pH 7.0. MGRS was composed of 2% (v/v) buffer solution (7.3% (w/v) Na(2)HPO(4), 3.2% (w/v) KH(2)PO(4), pH 7.2), 40 microg ml(-1) FeSO(4) and 40 microg ml(-1) MgSO(4), 0.1% (w/v) (NH(4))(2)SO(4) supplemented with 3% NaCl, 0.1% glucose, 1.0% rice bran oil and 0.5% (w/v) rice bran. ...
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The goal of this research was to isolate and identify the thermostable alkaline protease producing bacteria among several native Iranian microorganisms. At the end of screening program, a Bacillus subtilis BP-36 strain producing thermophilic alkaline protease was isolated from a hot spring in Ardebil province. The target enzyme was purified using a one-step Aqueous two-phase systems (ATPS) prot...
متن کاملProduction, Purification and Characterization of an Alkaline Protease from an Alkaliphilic Bacillus Sp
An extracellular serine like alkaline protease was purified and characterized from alkaliphilic Bacillus sp. The enzyme was purified to the homogeneity by a combination of aqueous two-phase system and affinity chromatographic techniques. The apparent molecular weight was estimated as 26-29 kDa based on SDS-PAGE and gel filtration chromatography and the enzyme appeared to be a monomer. The Km an...
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ژورنال
عنوان ژورنال: Chemical and Biochemical Engineering Quarterly
سال: 2017
ISSN: 0352-9568,1846-5153
DOI: 10.15255/cabeq.2016.99